Single-cell sequencing analysis reveals the molecular mechanism of promotion of SCAP proliferation upon AZD2858 treatment

The Wnt/β-catenin signaling pathway is the main target of tooth regeneration regulation. Treatment cells with AZD2858 stimulates pathway, yet function this in remains unclear. Here, we found that promotes accumulation β-catenin nuclei stem from apical papilla (SCAPs) and enhances cell proliferation. Single-cell sequencing was performed on SCAPs treated AZD2858. Eight clusters were identified, namely SCAPs-CNTNAP2, SCAPs-DTL, SCAPs-MYH11, SCAPs-MKI67, SCAPs-CXCL8, SCAPs-TPM2, SCAPs-IFIT2 SCAPs-NEK10. pseudo-time trajectory analysis showed enhanced evolution SCAPs-TMP2 to SCAPs-CNTNAPs SCAPs-NEK10 via up-regulation PRKCA, SMURF2, MAGI2, RBMS3, EXT1, CAMK2D, PLCB4, PLCB1. These results demonstrate proliferation SCAPs-TPM2 cluster by activating non-canonical pathway.